Our appologies for the recent down-time which was caused by the reconfiguration of our server. We also regret to inform that during this reconfiguration all the data stored in the MySQL database has been lost, this includes the registration information and the saved visualisations. Users who had been registered will need to reregister.
Abnormalities in DNA methylation of CpG islands that play a role in gene regulation affect gene expression and hence play a role in disease, including cancer. Bisulfite-based DNA methylation analysis methods such as methylation-specific PCR (MSP) and bisulfite sequencing are commonly used to study promoter DNA methylation. Importantly, visualizing the position of the PCR primers in the context of the CpG island and the promoter region of the gene is a laborious and tedious task, amongst others due to the sequence changes induced during the bisulfite conversion.
For this purpose, we designed methGraph, a web application developed for easy, fast and flexible visualization of any PCR-based methylation assay. A manual on how to use the tool can be found here.
You can login here to use methGraph.